Genotypic Characterization of Vibrio vulnificus Clinical Isolates in Korea

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Genotypic Characterization of Vibrio vulnificus Clinical Isolates in Korea

OBJECTIVES Vibrio vunificus is known to cause septicemia and severe wound infections in patients with chronic liver diseases or an immuno-compromised condition. We carried out the molecular characterization of V. vulnificus isolates from human Vibrio septicemia cases based on pulsed-field gel electrophoresis (PFGE) using NotI and SfiI. METHODS AND RESULTS PFGE was used to characterize a total...

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Detection and Characterization of Vibrio vulnificus and Vibrio parahaemolyticus Isolates: Pentaplex PCR Assay and its Application

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Genotypic Diversity and Population Structure of Vibrio vulnificus Strains Isolated in Taiwan and Korea as Determined by Multilocus Sequence Typing

The genetic diversity and population structure of Vibrio vulnificus isolates from Korea and Taiwan were investigated using PCR-based assays targeting putative virulence-related genes and multilocus sequence typing (MLST). BOX-PCR genomic fingerprinting identified 52 unique genotypes in 84 environmental and clinical V. vulnificus isolates. The majority (> 50%) of strains had pathogenic genotypes...

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We subtyped 117 Vibrio vulnificus isolates from 85 V. vulnificus sepsis patients and 32 environmental samples by performing automated ribotyping for the purpose of molecular epidemiological study. Although there was one indistinguishable ribotype among the four human isolates and one environmental isolate, taken as a whole, the ribotypes were highly diverse regardless of sample sources. We repo...

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Pyrosequencing-Based Comparative Genome Analysis of Vibrio vulnificus Environmental Isolates

Between 1996 and 2006, the US Centers for Disease Control reported that the only category of food-borne infections increasing in frequency were those caused by members of the genus Vibrio. The gram-negative bacterium Vibrio vulnificus is a ubiquitous inhabitant of estuarine waters, and is the number one cause of seafood-related deaths in the US. Many V. vulnificus isolates have been studied, an...

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ژورنال

عنوان ژورنال: Osong Public Health and Research Perspectives

سال: 2011

ISSN: 2210-9099

DOI: 10.1016/j.phrp.2011.04.008